primary antibodies for ednrb (Novus Biologicals)
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Novus Biologicals
primary antibodies for ednrb
Primary Antibodies For Ednrb, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary antibodies for ednrb/product/Novus Biologicals
Average 92 stars, based on 4 article reviews
Primary Antibodies For Ednrb, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary antibodies for ednrb/product/Novus Biologicals
Average 92 stars, based on 4 article reviews
primary antibodies for ednrb - by Bioz Stars,
2026-05
92/100 stars
Images
1) Product Images from "Endothelin 3/EDNRB signaling induces thermogenic differentiation of white adipose tissue"
Article Title: Endothelin 3/EDNRB signaling induces thermogenic differentiation of white adipose tissue
Journal: Nature Communications
doi: 10.1038/s41467-024-51579-0
Figure Legend Snippet: a – d Human white preadipocytes were pretreated with vehicle (Veh) or 100 nM EDN3 and with or without inhibitors for 3 days, followed by a 12-day adipogenesis. a Schematic of pretreatment experiments. UCP1 mRNA ( b ) and protein ( c ) levels in mature adipocytes (EV and EV and EDNRB OE). d UCP1 mRNA levels in mature adipocytes (EV and EDNRB KO). e Cytosolic calcium levels in human white preadipocytes after 100 nM EDN3 treatment. f , g Intracellular cAMP levels ( f ) and oxygen consumption rate (OCR; g ) in human white preadipocytes after 6 hours of Veh or EDN3 treatment. Maximal OCR was quantified in the right panel. h – j EV and EDNRB OE preadipocytes were pretreated with Veh or EDN3 combined with or without melittin (ME) or YM254890 (YM) for 3 days, followed by a 12-day adipogenesis. UCP1 mRNA ( h ) and protein ( i ) levels in mature adipocytes pretreated with ME. j UCP1 mRNA levels in mature adipocytes pretreated with YM. k The protein levels in preadipocytes after 6 hr of Veh or EDN3 treatment. The quantification of protein bands was in the right panels. l The EPAC1 protein in EDNRB OE preadipocytes after 48 hr of EPAC1 siRNA treatment. m EDNRB OE preadipocytes with or without EPAC1 knockdown were pretreated with Veh or EDN3 for 3 days, followed by a 12-day adipogenesis. Protein levels in mature adipocytes. n EV or EDNRB OE preadipocytes were pretreated with Veh, 1 μM or 10 μM of PD98059 (PD-1 or PD-10) for 3 days and were differentiated to mature adipocytes. UCP1 and DIO2 mRNA levels in human white adipocytes. n = 3 biological replicates/group in ( b – e ), ( i ), ( k – n ); n = 4 biological replicates/group in ( f – h ), ( j ). Data are presented as mean ± SEM. P values were determined using unpaired two-tailed t tests: ( l ); one-way ANOVA with Tukey’s multiple-comparison test: ( b – d ), ( f – k ), ( m ), ( n ); two-way ANOVA with Bonferroni’s multiple-comparison test: ( e ). Source data are provided as a Source Data file. Panel ( a ) was created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.
Techniques Used: Knockdown, Two Tailed Test, Comparison
Figure Legend Snippet: a , b Male mice were intraperitoneally injected by tamoxifen 5 times within 7 days to induce EDNRB KO in PDGFRα + preadipocytes (iKO) and were then housed at 5 °C for 7 days. a The mRNA levels of thermogenic genes in scWAT. CTL: n = 5, iKO: n = 6. b The protein levels of UCP1 in scWAT. The quantification of protein bands in right panel. n = 4 mice/group. c , d Control and iKO mice were housed at thermoneutral temperature (30 °C, TN) or cold (5 °C) for 7 days. Images of immunohistochemical stain for UCP1 proteins (UCP1 IHC; c ) and HE stain ( d ) in scWAT. High magnification images were in the right panels. Scale bar=100 μm. Two independent experiments were repeated with similar results, as shown in Supplementary Fig. a and . e The phosphorylated ERK and total ERK protein in scWAT of mice after 7 days of cold exposure. The quantification of protein bands in right panel. n = 3 mice/group. f , g PDGFRα + cell lineage-tracing control and EDNRB iKO mice were housed at thermoneutral temperature (30 °C, TN) or cold (5°C) for 7 days. f The immunofluorescent images from the section of scWAT. Scale bar=100 μm. g Quantification of GFP signal in each section of scWAT. CTL at TN: n = 6, iKO at TN: n = 6, CTL at Cold: n = 6, iKO at Cold: n = 7, 4 slides/mouse. h The mRNA levels of Ednrb, Pdgfrα and Ebf2 in SVF of scWAT from mice housed at TN or cold for 7 days. CTL at TN: n = 3, iKO at TN: n = 3, CTL at Cold: n = 3, iKO at Cold: n = 4. Data are presented as mean ± SEM. P values were determined using unpaired two-tailed t tests: ( a) , ( b ), ( e ); one-way ANOVA with Tukey’s multiple-comparison test: ( g ), ( h ). Source data are provided as a Source Data file.
Techniques Used: Injection, Control, Immunohistochemical staining, Staining, H&E Stain, Two Tailed Test, Comparison
